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fab9577b  (R&D Systems)


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    R&D Systems fab9577b
    Fab9577b, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fab9577b/Human+EGFR+(Research+Grade+Cetuximab+Biosimilar)+Biotinylated+Antibody/pm41579862-796-120-117
    Average 94 stars, based on 12 article reviews
    fab9577b - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Microarray:

    Article Title: Pan-cancer N-glycoproteomic atlas of patient-derived xenografts uncovers FAT2 as an actionable surface target.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER PDK1 Rabbit polyclonal antibody Cell Signaling Technology Cat# 3062; RRID:AB_2236832 Phospho-PDK1 (Ser241) (C49H2) Rabbit monoclonal antibody Cell Signaling Technology Cat# 3438; RRID:AB_2161134 PI3 Kinase p85 (19H8) Rabbit monoclonal antibody Cell Signaling Technology Cat# 4257; RRID:AB_659889 Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) (E3U1H) Rabbit monoclonal antibody Cell Signaling Technology Cat# 17366; RRID:AB_2895293 PRAS40 (D23C7) XP(tm) Rabbit monoclonal antibody Cell Signaling Technology Cat# 2691; RRID:AB_2225033 Phospho-PRAS40 (Thr246) (C77D7) Rabbit monoclonal antibody Cell Signaling Technology Cat# 2997; RRID:AB_2258110 Src (32G6) Rabbit monoclonal antibody Cell Signaling Technology Cat# 2123; RRID:AB_2106047 Phospho-Src Family (Tyr416) (D49G4) Rabbit monoclonal antibody Cell Signaling Technology Cat# 6943; RRID:AB_10013641 EGFR (D38B1) Rabbit monoclonal antibody Cell Signaling Technology Cat# 4267; RRID:AB_2246311 Anti-Human EGFR antibody R&D systems Cat# FAB9577B; RRID:AB_2942016 PE-Cy TM 7 Mouse Anti-Human CD3 antibody BD Biosciences Cat# #563423; RRID:AB_2738196 APC Mouse Anti-Human CD69 antibody BD Biosciences Cat# 555533; RRID:AB_398602 BV421 TM Anti-Human CD25 Antibody BioLegend Cat# #356114; RRID:AB_2562164 Biological samples Human normal tissue microarray Novus Biologicals Cat# NBP2-78113 Human paraffin-embedded normal cerebellum tissue BioChain Cat# T2234039 Human paraffin-embedded normal liver tissue BioChain Cat# T2234149 Chemicals, peptides, and recombinant proteins UltraLink TM Hydrazide Resin Thermo Cat# 53149 SiMAG-Hydrazide beads Chemicell Cat# 1404-5 Biocytin hydrazide Biotium Cat# 90060 N-Glycosidase F Roche Cat# 06538355103 Sera-Mag TM Carboxylate-Modified Magnetic Beads & SpeedBeads Cytiva Cat# 45152105050250 and Cat# 65152105050250 Streptavidin Sepharose TM High Performance beads GE HealthCare Cat# 17-5113-01 Trypsin/Lys-C Mix, Mass Spec Grade Promega Cat# V5071 Critical commercial assays Human IFN-gamma DuoSet ELISA R&D Systems Cat# DY285B Human TNF-alpha DuoSet ELISA R&D Systems Cat# DY210 Deposited data Raw mass spectrometry data This study UCSD’s MassIVE database: #MSV000096571. .. Processed mass spectrometry data This study Data S1, S2, and S3 and available at http://kislingerlab.uhnres.utoronto.ca/ glycoPDXplorer/ Experimental models: Cell lines Human Oral Epithelial Primary Cell Culture Celprogen Cat# 36063-01 HT-29 ATCC Cat# HTB-38; RRID:CVCL_0320 FaDu ATCC Cat# HTB-43; RRID:CVCL_1218 (Continued on next page) Cell Reports Medicine 7, 102578, February 17, 2026 e2 OPEN ACCESS

    Recombinant:

    Article Title: Pan-cancer N-glycoproteomic atlas of patient-derived xenografts uncovers FAT2 as an actionable surface target.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER PDK1 Rabbit polyclonal antibody Cell Signaling Technology Cat# 3062; RRID:AB_2236832 Phospho-PDK1 (Ser241) (C49H2) Rabbit monoclonal antibody Cell Signaling Technology Cat# 3438; RRID:AB_2161134 PI3 Kinase p85 (19H8) Rabbit monoclonal antibody Cell Signaling Technology Cat# 4257; RRID:AB_659889 Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) (E3U1H) Rabbit monoclonal antibody Cell Signaling Technology Cat# 17366; RRID:AB_2895293 PRAS40 (D23C7) XP(tm) Rabbit monoclonal antibody Cell Signaling Technology Cat# 2691; RRID:AB_2225033 Phospho-PRAS40 (Thr246) (C77D7) Rabbit monoclonal antibody Cell Signaling Technology Cat# 2997; RRID:AB_2258110 Src (32G6) Rabbit monoclonal antibody Cell Signaling Technology Cat# 2123; RRID:AB_2106047 Phospho-Src Family (Tyr416) (D49G4) Rabbit monoclonal antibody Cell Signaling Technology Cat# 6943; RRID:AB_10013641 EGFR (D38B1) Rabbit monoclonal antibody Cell Signaling Technology Cat# 4267; RRID:AB_2246311 Anti-Human EGFR antibody R&D systems Cat# FAB9577B; RRID:AB_2942016 PE-Cy TM 7 Mouse Anti-Human CD3 antibody BD Biosciences Cat# #563423; RRID:AB_2738196 APC Mouse Anti-Human CD69 antibody BD Biosciences Cat# 555533; RRID:AB_398602 BV421 TM Anti-Human CD25 Antibody BioLegend Cat# #356114; RRID:AB_2562164 Biological samples Human normal tissue microarray Novus Biologicals Cat# NBP2-78113 Human paraffin-embedded normal cerebellum tissue BioChain Cat# T2234039 Human paraffin-embedded normal liver tissue BioChain Cat# T2234149 Chemicals, peptides, and recombinant proteins UltraLink TM Hydrazide Resin Thermo Cat# 53149 SiMAG-Hydrazide beads Chemicell Cat# 1404-5 Biocytin hydrazide Biotium Cat# 90060 N-Glycosidase F Roche Cat# 06538355103 Sera-Mag TM Carboxylate-Modified Magnetic Beads & SpeedBeads Cytiva Cat# 45152105050250 and Cat# 65152105050250 Streptavidin Sepharose TM High Performance beads GE HealthCare Cat# 17-5113-01 Trypsin/Lys-C Mix, Mass Spec Grade Promega Cat# V5071 Critical commercial assays Human IFN-gamma DuoSet ELISA R&D Systems Cat# DY285B Human TNF-alpha DuoSet ELISA R&D Systems Cat# DY210 Deposited data Raw mass spectrometry data This study UCSD’s MassIVE database: #MSV000096571. .. Processed mass spectrometry data This study Data S1, S2, and S3 and available at http://kislingerlab.uhnres.utoronto.ca/ glycoPDXplorer/ Experimental models: Cell lines Human Oral Epithelial Primary Cell Culture Celprogen Cat# 36063-01 HT-29 ATCC Cat# HTB-38; RRID:CVCL_0320 FaDu ATCC Cat# HTB-43; RRID:CVCL_1218 (Continued on next page) Cell Reports Medicine 7, 102578, February 17, 2026 e2 OPEN ACCESS

    Magnetic Beads:

    Article Title: Pan-cancer N-glycoproteomic atlas of patient-derived xenografts uncovers FAT2 as an actionable surface target.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER PDK1 Rabbit polyclonal antibody Cell Signaling Technology Cat# 3062; RRID:AB_2236832 Phospho-PDK1 (Ser241) (C49H2) Rabbit monoclonal antibody Cell Signaling Technology Cat# 3438; RRID:AB_2161134 PI3 Kinase p85 (19H8) Rabbit monoclonal antibody Cell Signaling Technology Cat# 4257; RRID:AB_659889 Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) (E3U1H) Rabbit monoclonal antibody Cell Signaling Technology Cat# 17366; RRID:AB_2895293 PRAS40 (D23C7) XP(tm) Rabbit monoclonal antibody Cell Signaling Technology Cat# 2691; RRID:AB_2225033 Phospho-PRAS40 (Thr246) (C77D7) Rabbit monoclonal antibody Cell Signaling Technology Cat# 2997; RRID:AB_2258110 Src (32G6) Rabbit monoclonal antibody Cell Signaling Technology Cat# 2123; RRID:AB_2106047 Phospho-Src Family (Tyr416) (D49G4) Rabbit monoclonal antibody Cell Signaling Technology Cat# 6943; RRID:AB_10013641 EGFR (D38B1) Rabbit monoclonal antibody Cell Signaling Technology Cat# 4267; RRID:AB_2246311 Anti-Human EGFR antibody R&D systems Cat# FAB9577B; RRID:AB_2942016 PE-Cy TM 7 Mouse Anti-Human CD3 antibody BD Biosciences Cat# #563423; RRID:AB_2738196 APC Mouse Anti-Human CD69 antibody BD Biosciences Cat# 555533; RRID:AB_398602 BV421 TM Anti-Human CD25 Antibody BioLegend Cat# #356114; RRID:AB_2562164 Biological samples Human normal tissue microarray Novus Biologicals Cat# NBP2-78113 Human paraffin-embedded normal cerebellum tissue BioChain Cat# T2234039 Human paraffin-embedded normal liver tissue BioChain Cat# T2234149 Chemicals, peptides, and recombinant proteins UltraLink TM Hydrazide Resin Thermo Cat# 53149 SiMAG-Hydrazide beads Chemicell Cat# 1404-5 Biocytin hydrazide Biotium Cat# 90060 N-Glycosidase F Roche Cat# 06538355103 Sera-Mag TM Carboxylate-Modified Magnetic Beads & SpeedBeads Cytiva Cat# 45152105050250 and Cat# 65152105050250 Streptavidin Sepharose TM High Performance beads GE HealthCare Cat# 17-5113-01 Trypsin/Lys-C Mix, Mass Spec Grade Promega Cat# V5071 Critical commercial assays Human IFN-gamma DuoSet ELISA R&D Systems Cat# DY285B Human TNF-alpha DuoSet ELISA R&D Systems Cat# DY210 Deposited data Raw mass spectrometry data This study UCSD’s MassIVE database: #MSV000096571. .. Processed mass spectrometry data This study Data S1, S2, and S3 and available at http://kislingerlab.uhnres.utoronto.ca/ glycoPDXplorer/ Experimental models: Cell lines Human Oral Epithelial Primary Cell Culture Celprogen Cat# 36063-01 HT-29 ATCC Cat# HTB-38; RRID:CVCL_0320 FaDu ATCC Cat# HTB-43; RRID:CVCL_1218 (Continued on next page) Cell Reports Medicine 7, 102578, February 17, 2026 e2 OPEN ACCESS

    Mass Spectrometry:

    Article Title: Pan-cancer N-glycoproteomic atlas of patient-derived xenografts uncovers FAT2 as an actionable surface target.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER PDK1 Rabbit polyclonal antibody Cell Signaling Technology Cat# 3062; RRID:AB_2236832 Phospho-PDK1 (Ser241) (C49H2) Rabbit monoclonal antibody Cell Signaling Technology Cat# 3438; RRID:AB_2161134 PI3 Kinase p85 (19H8) Rabbit monoclonal antibody Cell Signaling Technology Cat# 4257; RRID:AB_659889 Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) (E3U1H) Rabbit monoclonal antibody Cell Signaling Technology Cat# 17366; RRID:AB_2895293 PRAS40 (D23C7) XP(tm) Rabbit monoclonal antibody Cell Signaling Technology Cat# 2691; RRID:AB_2225033 Phospho-PRAS40 (Thr246) (C77D7) Rabbit monoclonal antibody Cell Signaling Technology Cat# 2997; RRID:AB_2258110 Src (32G6) Rabbit monoclonal antibody Cell Signaling Technology Cat# 2123; RRID:AB_2106047 Phospho-Src Family (Tyr416) (D49G4) Rabbit monoclonal antibody Cell Signaling Technology Cat# 6943; RRID:AB_10013641 EGFR (D38B1) Rabbit monoclonal antibody Cell Signaling Technology Cat# 4267; RRID:AB_2246311 Anti-Human EGFR antibody R&D systems Cat# FAB9577B; RRID:AB_2942016 PE-Cy TM 7 Mouse Anti-Human CD3 antibody BD Biosciences Cat# #563423; RRID:AB_2738196 APC Mouse Anti-Human CD69 antibody BD Biosciences Cat# 555533; RRID:AB_398602 BV421 TM Anti-Human CD25 Antibody BioLegend Cat# #356114; RRID:AB_2562164 Biological samples Human normal tissue microarray Novus Biologicals Cat# NBP2-78113 Human paraffin-embedded normal cerebellum tissue BioChain Cat# T2234039 Human paraffin-embedded normal liver tissue BioChain Cat# T2234149 Chemicals, peptides, and recombinant proteins UltraLink TM Hydrazide Resin Thermo Cat# 53149 SiMAG-Hydrazide beads Chemicell Cat# 1404-5 Biocytin hydrazide Biotium Cat# 90060 N-Glycosidase F Roche Cat# 06538355103 Sera-Mag TM Carboxylate-Modified Magnetic Beads & SpeedBeads Cytiva Cat# 45152105050250 and Cat# 65152105050250 Streptavidin Sepharose TM High Performance beads GE HealthCare Cat# 17-5113-01 Trypsin/Lys-C Mix, Mass Spec Grade Promega Cat# V5071 Critical commercial assays Human IFN-gamma DuoSet ELISA R&D Systems Cat# DY285B Human TNF-alpha DuoSet ELISA R&D Systems Cat# DY210 Deposited data Raw mass spectrometry data This study UCSD’s MassIVE database: #MSV000096571. .. Processed mass spectrometry data This study Data S1, S2, and S3 and available at http://kislingerlab.uhnres.utoronto.ca/ glycoPDXplorer/ Experimental models: Cell lines Human Oral Epithelial Primary Cell Culture Celprogen Cat# 36063-01 HT-29 ATCC Cat# HTB-38; RRID:CVCL_0320 FaDu ATCC Cat# HTB-43; RRID:CVCL_1218 (Continued on next page) Cell Reports Medicine 7, 102578, February 17, 2026 e2 OPEN ACCESS

    Enzyme-linked Immunosorbent Assay:

    Article Title: Pan-cancer N-glycoproteomic atlas of patient-derived xenografts uncovers FAT2 as an actionable surface target.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER PDK1 Rabbit polyclonal antibody Cell Signaling Technology Cat# 3062; RRID:AB_2236832 Phospho-PDK1 (Ser241) (C49H2) Rabbit monoclonal antibody Cell Signaling Technology Cat# 3438; RRID:AB_2161134 PI3 Kinase p85 (19H8) Rabbit monoclonal antibody Cell Signaling Technology Cat# 4257; RRID:AB_659889 Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) (E3U1H) Rabbit monoclonal antibody Cell Signaling Technology Cat# 17366; RRID:AB_2895293 PRAS40 (D23C7) XP(tm) Rabbit monoclonal antibody Cell Signaling Technology Cat# 2691; RRID:AB_2225033 Phospho-PRAS40 (Thr246) (C77D7) Rabbit monoclonal antibody Cell Signaling Technology Cat# 2997; RRID:AB_2258110 Src (32G6) Rabbit monoclonal antibody Cell Signaling Technology Cat# 2123; RRID:AB_2106047 Phospho-Src Family (Tyr416) (D49G4) Rabbit monoclonal antibody Cell Signaling Technology Cat# 6943; RRID:AB_10013641 EGFR (D38B1) Rabbit monoclonal antibody Cell Signaling Technology Cat# 4267; RRID:AB_2246311 Anti-Human EGFR antibody R&D systems Cat# FAB9577B; RRID:AB_2942016 PE-Cy TM 7 Mouse Anti-Human CD3 antibody BD Biosciences Cat# #563423; RRID:AB_2738196 APC Mouse Anti-Human CD69 antibody BD Biosciences Cat# 555533; RRID:AB_398602 BV421 TM Anti-Human CD25 Antibody BioLegend Cat# #356114; RRID:AB_2562164 Biological samples Human normal tissue microarray Novus Biologicals Cat# NBP2-78113 Human paraffin-embedded normal cerebellum tissue BioChain Cat# T2234039 Human paraffin-embedded normal liver tissue BioChain Cat# T2234149 Chemicals, peptides, and recombinant proteins UltraLink TM Hydrazide Resin Thermo Cat# 53149 SiMAG-Hydrazide beads Chemicell Cat# 1404-5 Biocytin hydrazide Biotium Cat# 90060 N-Glycosidase F Roche Cat# 06538355103 Sera-Mag TM Carboxylate-Modified Magnetic Beads & SpeedBeads Cytiva Cat# 45152105050250 and Cat# 65152105050250 Streptavidin Sepharose TM High Performance beads GE HealthCare Cat# 17-5113-01 Trypsin/Lys-C Mix, Mass Spec Grade Promega Cat# V5071 Critical commercial assays Human IFN-gamma DuoSet ELISA R&D Systems Cat# DY285B Human TNF-alpha DuoSet ELISA R&D Systems Cat# DY210 Deposited data Raw mass spectrometry data This study UCSD’s MassIVE database: #MSV000096571. .. Processed mass spectrometry data This study Data S1, S2, and S3 and available at http://kislingerlab.uhnres.utoronto.ca/ glycoPDXplorer/ Experimental models: Cell lines Human Oral Epithelial Primary Cell Culture Celprogen Cat# 36063-01 HT-29 ATCC Cat# HTB-38; RRID:CVCL_0320 FaDu ATCC Cat# HTB-43; RRID:CVCL_1218 (Continued on next page) Cell Reports Medicine 7, 102578, February 17, 2026 e2 OPEN ACCESS



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    (A) Genes were all highly expressed in two cancer cell lines and barely expressed in BM samples. Specifically, there were no detectable levels of EGFR and EpCAM in both BM samples. MDA: MDA-MB-231; MCF7: MCF-7; BM1, BM2: bone marrow cells from healthy donors #1 and #2; BM1+1, +5, +50: 1, 5, 50 MDA-MB-231 cells were spiked into 1 million BM cells from healthy donor #1. (B) EGFR was highly expressed in MDA-MB-231 cells, an observation that aligned with the literature. Nucleated human BM cells were used as control. Streptavidin-PE was used to label biotinylated antibodies.

    Journal: PLOS One

    Article Title: Microfluidic isolation and release of live disseminated breast tumor cells in bone marrow

    doi: 10.1371/journal.pone.0319392

    Figure Lengend Snippet: (A) Genes were all highly expressed in two cancer cell lines and barely expressed in BM samples. Specifically, there were no detectable levels of EGFR and EpCAM in both BM samples. MDA: MDA-MB-231; MCF7: MCF-7; BM1, BM2: bone marrow cells from healthy donors #1 and #2; BM1+1, +5, +50: 1, 5, 50 MDA-MB-231 cells were spiked into 1 million BM cells from healthy donor #1. (B) EGFR was highly expressed in MDA-MB-231 cells, an observation that aligned with the literature. Nucleated human BM cells were used as control. Streptavidin-PE was used to label biotinylated antibodies.

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